Massachusetts Institute of Technology;Centre national de la recherche scientifique;ENSCM;UNIVERSITE DE MONTPELLIER
发明人:
Barbara Imperiali,Juan Antonio Gonzalez Vera
申请号：
US16299976
公开号：
US20190275172A1
申请日:
2019.03.12
申请国别(地区):
US
年份:
2019
代理人:
摘要：
The present invention generally relates to assays of enzymes responsible for phosphoregulation including kinases (such as protein kinases, which mediate protein and peptide phosphorylation) and phosphatases (such as protein phosphatases, which mediate protein and peptide dephosphorylation). Certain aspects of the invention use lanthanide ions such as europium ions that exhibit chelation-enhanced luminescence. Phosphorylation of a peptide by a kinase may cause a complex to form between the lanthanide ion, the phosphate group, and a reporter group such as a hydroxyquinoline, which results in luminescence when in the complexed state. Thus, in certain embodiments, determination of luminescence may be indicative of kinase activity. Certain embodiments also include the use of substrates for detection of phosphatase activity, where dephosphorylation results in a loss of signal assay. Other aspects are generally related to techniques for making and using such peptides or complexes, kits involving such peptides or complexes, and the like.
