The present invention relates to methods of wound debridement. Particularly, the present invention relates to methods of debridement of chronic wounds comprising topically applying to a wound site a debriding formulation in the form of a hydrogel comprising a proteolytic enzyme mixture obtained from bromelain and a water-soluble gelling agent, the debriding formulation being applied to the wound site up to ten times over a period of up to four weeks, thereby achieving debridement of chronic wounds.