DAVIS, BENJAMIN G.,JONES, JOHN BRYAN,BOTT, RICHARD R.
申请号：
CA2443069
公开号：
CA2443069C
申请日:
2002.04.02
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
The present invention relates to a chemically modified mutant proteinincluding a cysteine residue substituted for a residue other than cysteine n aprecursor protein, the substituted cysteine residue being subsequentlymodified by reacting the cysteine residue with a glycosylated thiosulfonate.Also a method of producing the chemically modified mutant protein is provided.The present invention also relates to a glycosylated methanethiosulfonate.Another aspect of the present invention is a method of modifying thefunctional characteristics of a protein including providing a protein andreacting the protein with a glycosylated methanethiosulfonate reagent underconditions effective to produce a glycoprotein with altered functionalcharacteristics as compared to the protein. In addition, the present inventionrelates to methods of determining the structure-function relationships ofchemically modified mutant proteins. The present invention also relates tosynthetic methods for producing thio-glycoses, the thio-glycoses so produced,and to methods for producing glycodendrimer reagents.
