BARRANGOU, RODOLPHE,FREMAUX, CHRISTOPHE,HORVATH, PHILIPPE,ROMERO, DENNIS,BOYAVAL, PATRICK
申请号：
ES08726285
公开号：
ES2541693T3
申请日:
2008.02.29
申请国别(地区):
ES
年份:
2015
代理人:
摘要：
A method for generating a starter culture comprising at least two variants of a bacteriophage resistant strain, comprising the steps of: (a) the exposure of a precursor bacterial strain comprising at least a portion of a CRISPR location to a bacteriophage to produce a mixture of bacteria comprising a variant of the bacteriophage resistant strain comprising a modified CRISPR location comprising at least one additional spacer at said modified CRISPR location； (b) independent exposure of the same precursor bacterial strain as in step (a) comprising at least a portion of a CRISPR location to the same bacteriophage as in step (a) to produce a mixture of bacteria comprising another variant of the bacteriophage resistant strain comprising a modified CRISPR location comprising at least one additional spacer at said modified CRISPR location； (c) the selection of said variants of the bacteriophage resistant strain of said bacterial mixtures； (d) the selection of said variants of the bacteriophage resistant strain comprising an additional spacer at said modified CRISPR location of said bacteriophage resistant strains selected in step (c)； and (e) isolating said variants of the bacteriophage resistant strain, wherein said strains comprise an additional spacer at said modified CRISPR location； where the sequence of at least one additional spacer in the variant of the bacteriophage resistant strain is different from the sequence of at least one additional spacer in the other bacteriophage resistant strain.Un método para generar un cultivo de inicio que comprende al menos dos variantes de una cepa resistente a bacteriófagos, que comprende los pasos de: (a) la exposición de una cepa bacteriana precursora que comprende al menos una porción de una ubicación CRISPR a un bacteriófago para producir una mezcla de bacterias que comprende una variante de la cepa resistente a bacteriófagos que comprende una ubicación CRISPR modificada que comprende al menos un espaciador adicional en dicha
