The present invention provides a composition for preventing, ameliorating, alleviating or treating dermatitis comprising exosomes derived from adipose-derived stem cells as an active ingredient. The composition of the present invention is able to act against dermatitis-inducing multiple cytokine targets, and thus be widely applied against dermatitis caused by various factors and effectively suppress and alleviate dermatitis. In addition, the composition of the present invention can protect and strength the skin barrier to decrease water evaporation from stratum corneum and to improve skin hydration, and prevent, ameliorate, alleviate or treat dermatitis through this skin barrier protection and strengthening.
