Methods are provided for producing liposomes, including a first mixing step of mixing one or more lipids and an aqueous solution containing a water-miscible organic solvent a heating step of heating the mixture of the lipids and the aqueous solution to a temperature at which the lipids are dissolved in the aqueous solution and, after the heating step, a cooling step of cooling the resulting mixture to a temperature at which liposomes are produced. By this method, liposomes with a uniform particle diameter and have a high encapsulation ratio can be easily obtained.
