The invention provides a Saccharomyces cerevisiae strain with unique multi-carbon-coutilizing characteristics and its application, relates to the field of microbiological technologies and fermentation engineering technology. S. cerevisiae CCTCC M 2014463 was screened from the Chinese Maotai-flavored liquor making environment. The strain can use glucose, galactose, xylose, alfa ketone, sucrose, maltose, honey disaccharide, loose sugar, trehalose, raffinose and it can use glucose and any of the sugars above simultaneously. In addition, the strain can endure high temperature, acid and alcohol, and is capable of producing a variety of flavor metabolites, including acids, alcohols and esters. The stain could be directly used in alcoholic beverage production, the enhancemment application of S. cerevisiae in alcoholic beverage production, and fuel ethanol production, and it can significantly improve the carbon source utilization and ethanol yield.
