PROBLEM TO BE SOLVED: To provide a method for quickly forming a medium for Tricholoma matsutake.SOLUTION: The fruit body production method comprises preparation of a carbon source culture liquid and a nitrogen culture liquid prepared by using a carbon source comprising a saccharide having a reducing terminal as a medium, a nitrogen source comprising amino acids having amino group, a metal salt and a gelling agent wherein the carbon source and the nitrogen source are separately prepared and adding the metal salt and the gelling agent to different sources introduction of mycelium of Tricholoma matsutake into one of the culture liquid and addition of the culture liquid containing the gelling agent to promote the gelation of the mixture system comprising the carbon source culture liquid and the nitrogen source culture liquid in the presence of air and cultivation of the obtained fungus body suspension gel in a dark chamber.COPYRIGHT: (C)2013,JPO&INPIT【課題】迅速にマツタケの培地を形成することが可能な方法を提供する。【解決手段】培地として還元末端を有する糖類である炭素源と、アミノ基を有するアミノ酸類である窒素源と、金属塩とゲル化剤とからなって、炭素源と窒素源とは別々調合して、金属塩とゲル化剤とをそれぞれ別々の源に加えて、炭素源培養液と窒素培養液を生成して、一方にマツタケ菌糸体を投入して、ゲル剤を含む培養液を添加して、炭素源培養液と窒素源培養液との混合系のゲル化を空気含有で促進させた菌体懸濁ゲルを暗室下で培養する。【選択図】なし