BHARGAVA, Swapnil,CHEN, Cheng-Wei Aaron,LEITH, Matthew J.
申请号：
EP17866605
公开号：
EP3534949A4
申请日:
2017.11.06
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
The present invention provides a method for removing cysteine caps from antibodies and re-capping the antibodies with cysteine molecules. The methods include, inter alia, culturing a host cell comprising a protein molecule having at least one capped engineered cysteine residue, and contacting the cell culture with cystine. Dissolved oxygen levels can be manipulated in the cell culture to further enhance the removal and re-capping process.
