Swapnil Bhargava,Cheng-Wei Aaron Chen,Matthew J. Leith
申请号：
US16347919
公开号：
US20190290777A1
申请日:
2017.11.06
申请国别(地区):
US
年份:
2019
代理人:
摘要：
The present invention provides a method for removing cysteine caps from antibodies and re-capping the antibodies with cysteine molecules. The methods include, inter alia, culturing a host cell comprising a protein molecule having at least one capped engineered cysteine residue, and contacting the cell culture with cystine. Dissolved oxygen levels can be manipulated in the cell culture to further enhance the removal and re-capping process.
