Disclosed is a method of inexpensively manufacturing high concentrations of zeaxanthin microbiologically, while reducing production of gluconic acid. Specifically disclosed is a method of manufacture for a carotenoid containing zeaxanthin, characterised by a step wherein a bacterium which produces a carotenoid containing zeaxanthin is cultivated in a culture medium containing biotin, and when compared to a culture solution after culturing in a culture medium not containing biotin, the produced concentration of gluconic acid in the culture solution after culturing is lower and the produced concentration of zeaxanthin is higher.