The invention provides a cryopreservation solution as well as a preparation method and an application thereof and belongs to the technical field of biomaterial cryopreservation. In the cryopreservation solution, graphene oxide is added, so that growth of tiny ice crystals during a recovery process of the cryopreservation solution can be inhibited, and further the destructive effect of ice crystals on cells during a growth process is avoided, so that the survival rate and cell activity of biomaterials subjected to cryopreservation recovery are greatly improved. The method aims at inhibiting growth of the ice crystals, so that the cryopreservation solution has relatively high universality, can be used for cryopreservation of cells, tissues and the like, and can meet the needs of actual use to the greatest extent.
