MACDONALD, LYNN,STEVENS, SEAN,GURER, CAGAN,MURPHY, ANDREW J.,HOSIAWA, KAROLINA A.
申请号：
RSP20160483
公开号：
RS54891B1
申请日:
2011.06.22
申请国别(地区):
RS
年份:
2016
代理人:
摘要：
Genetically modified mice are provided that express human » variable (hV»,) sequences, including mice that express hV» sequences from an endogenous mouse » light chain locus, mice that express hV», sequences from an endogenous mouse º light chain locus, and mice that express hV», sequences from a transgene or an episome wherein the hV», sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human » variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human » variable sequences, including human antibodies, are provided.Miš koji obuhvata endogeni λ (lambda) alel lakog lanca koji obuhvata nepreraspoređeni ljudski promenljivi lambda (hVλ) i nepreraspoređeni ljudski spojni (hJλ) segment gena, pri čemu miš obuhvata brisanje prve Vλ-Jλ-Cλ skupine gena pomenutog endogenog mišjeg λ alela lakog lanca i zamenu, u celini ili delimično, Vλ-Jλ, segmente gena druge Vλ-Jλ-Cλ skupine gena pomenutog alela sa Ijudskim VAi JX segmentima gena koji su funkcionalno povezani na nedirnuti mišji lambda konstantni (Cλ) region gena pomenute druge Vλ-Jλ-Cλ skupine gena tako da miš ispoljava laki lanac izveden izhVλ hJλ i mišji Cλ gen, pri čemu se endogeni pojačavači Enh 2.4, mišjeg lambda 3' pojačivača (Enh) i Enh 3.1 održavaju nedimuti u pomenutom λ alelu lakog lanca.Prijava sadrži još 16 patentnih zahteva.
