Provided is a method for manufacturing a cell mass containing olfactory nerve cells or its precursor cell which comprises the following steps (1) to (3): step (1) of culturing pluripotent stem cells in suspension in the presence of a first Wnt signal transduction pathway inhibitor to form cell aggregates, step (2) of culturing the cell aggregate obtained in the step (1) in suspension in the presence of a substance acting on a BMP signal transduction pathway, and step (3) of obtaining the cell mass by suspension culture of the cell aggregate obtained in the step (2), wherein step (3) comprises at least one step selected from the group consisting of step (3a) of culturing in suspension in the presence of an FGF signaling pathway agent, step (3b) of culturing in suspension in the presence of a BMP signaling pathway inhibitor (3b), and step (3c) of culturing in the presence of an FGF signaling pathway agonist and a BMP signaling pathway inhibitor.本發明係一種含有嗅神經細胞或其前驅細胞之細胞塊的製造方法，該製造方法包含下列步驟(1)至(3)：於第一Wnt訊息傳導路徑抑制物質之存在下懸浮培養多能性幹細胞，而形成細胞凝集物之步驟(1)；於BMP訊息傳導路徑作用物質之存在下懸浮培養前述步驟(1)所得到之細胞凝集物之步驟(2)；將前述步驟(2)所得到之細胞凝集物懸浮培養，而獲得前述細胞塊之步驟(3)，該步驟(3)含有選自由於FGF訊息傳導路徑作用物質之存在下做懸浮培養之步驟(3a)、於BMP訊息傳導路徑抑制物質之存在下做懸浮培養之步驟(3b)、以及於FGF訊息傳導路徑作用物質以及BMP訊息傳導路徑抑制物質之存在下做懸浮培養之步驟(3c)所組成的群組中之至少一個步驟。
