KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
发明人:
CHOI, CHUL HEEKR,최철희,YIM, NAM BINKR,임남빈,HEO, WON DOKR,허원도,RYU, SEUNG WOOKKR,류승욱,CHOI, HO JUNKR,최호준,CHOI, KYUNG SUNKR,최경선
申请号：
KR1020160055687
公开号：
KR1020160130949A
申请日:
2016.05.04
申请国别(地区):
KR
年份:
2016
代理人:
摘要：
The present invention relates to a method for massively manufacturing an exosome including a target protein to an exosome-producing vector which can be used to manufacture the exosome to an exosome including a target protein, which is manufactured by the method and to a method for delivering a target protein to cytoplasm by using the manufactured exosome. According to the present invention, in the case of using a manufacturing method of an exosome including a target protein, an exosome including a target protein can be manufactured at high yield, and thus can be widely used to treat diseases. The method for massively manufacturing an exosome including a target protein comprises the following steps: (a) introducing a polynucleotide which encodes a fusion protein composed of an exosome-specific marker and a target protein into an exosome-producing cell to obtain a transformed cell and (b) cultivating the transformed cell.COPYRIGHT KIPO 2016본 발명은 목적 단백질을 포함하는 엑소솜을 대량으로 제조하는 방법, 상기 엑소솜 제조에 사용될 수 있는 엑소솜 제조용 벡터, 상기 방법으로 제조된 목적 단백질을 포함하는 엑소솜 및 제조된 엑소솜을 이용하여 목적 단백질을 세포질로 전달시키는 방법에 관한 것이다. 본 발명에서 제공하는 목적 단백질을 포함하는 엑소솜의 제조방법을 이용하면, 목적 단백질을 포함하는 엑소솜을 높은 수율로 제조할 수 있으므로, 상기 엑소솜을 이용한 질환의 치료에 널리 활용될 수 있을 것이다.
