The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads;Georgia Tech Research Corporation;The Regents of the University of California
发明人:
Jessica Butts,Todd C. McDevitt
申请号：
US16303580
公开号：
US20200318065A1
申请日:
2017.05.26
申请国别(地区):
US
年份:
2020
代理人:
摘要：
Methods of generating spinal cord glutamatergic interneurons (V2a interneurons) from human pluripotent stem cells (hPSCs) are provided. A method of the present disclosure may include culturing a first population of hPSCs in vitro in a neural induction medium that includes: a retinoic acid signaling pathway activator； a sonic hedgehog (Shh) signaling pathway activator； and a Notch signaling pathway inhibitor, wherein the culturing results in generation of a second population of cultured cells containing CHX10+ V2a interneurons. Also provided are non-human animal models that include the hPSC-derived spinal cord glutamatergic interneurons, and methods of producing the non-human animal models.
