PURPOSE:To produce a cell containing antioxidative substance useful as a food and pharmaceutical, by inducing a reproductive cell from a grown sesame plant and culturing the cell in a medium. CONSTITUTION:Slices of sprout, stalk, leaf and/or root of aseptically prepared seedling of sesame are cultured in a solid and/or liquid medium containing carbon source, nitrogen source, vitamins, minerals, etc., and preferably added with phytohormones such as auxin, cytokinin, etc., in dark place at 28-30 deg.C and the induced reproductive callus obtained by this process is subcultured to form a large callus. A stably reproducible cell is grown from the callus and obtained cell is proliferated in a medium containing naphthaleneacetic acid, benzyladenine, gelan gum, etc., and added with natural organic nutrients in light place at 20-37 deg.C, preferably 25-35 deg.C and pH5.6-6.0. The objective antioxidative substance is separated from the proliferated cell.
