Общество с ограниченной ответственностью "СинБио" (RU)
发明人:
Автушенко Сергей Сергеевич (RU),Сурков Кирилл Геннадиевич (RU),Петров Андрей Валерьевич (RU),Ян Сергей Федорович (RU),Лазарев Вячеслав Сергеевич (RU),Гаврилова Евгения Николаевна (RU)
申请号：
RU2015152861
公开号：
RU2015152861A
申请日:
2015.12.09
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
The invention relates to medicine and concerns a method for isolating a protein containing insulin and one polysialic acid residue from reaction byproducts and unreacted starting material, by means of reverse-phase chromatography. The method employs the chromatographic material PLRP-S equilibrated with a solution comprising a non-polar component in an amount of 2-8%, and a stepwise gradient elution. The invention provides for a high-purity protein in one isolation step.
