The present invention, of intractable infectious diseases such as onychomycosis, which enables was difficult to conventional manufacturing, the production of animal infection models of microbial infection of the tissues that are difficult to infections, such as nails, and using the animal model of infection and provides a useful evaluation method for the development of therapeutic agents. To produce an animal model of infection tissue infection causative organism is difficult to infection by after previously administered to the animal immunosuppressive components were allowed to reduce the immunity and infected by inoculating the infection causative organism, reproduced It can be produced in a short period of time, reflecting the clinical pathology and good sex, animal models of infection caused microbial infections.本発明は、従来作製が困難であった、爪などの感染しにくい組織の微生物感染症の動物感染モデルの作製を可能とし、および該動物感染モデルを用いた爪白癬などの難治性感染症の治療薬の開発に有用な評価方法を提供する。感染症原因微生物が感染しにくい組織の動物感染モデルを作製するため、免疫抑制成分を動物に予め投与し、免疫能を低下せしめた後、感染症原因微生物を接種して感染させることにより、再現性がよくかつ臨床病態を反映した、感染症原因微生物の動物感染モデルを、短期間で作製することができる。
