PURPOSE: A freeze-drying method for photorhabdus temperata M1021 (deposit number: kacc91627p) and freeze-drying powder are provided to increase survival rate of photorhabdus temperate M1021 strain and to facilitate formulation.CONSTITUTION: A freeze-drying method for photorhabdus temperata M1021 (deposit number: kacc91627p) is processed by adding skim milk, sodium alginate, starch, or preserver of glucose. The skim milk is added in 1-10% concentration. The skim milk is added in 7 % concentration. The sodium alginate is added in 0.5-2% concentration. The starch is added in 1-7 % concentration. The glucose is added in 0.5-1.5% concentration. The freeze-drying is processed at -60 to -80 deg. Celsius for 48-72 hours. The biological agricultural chemical includes freeze-drying powder of photorhabdus temperata M1021 strain. The biological agricultural chemical is the agricultural chemical for Spodoptera frugiperda prevention.COPYRIGHT KIPO 2013본 발명은 스킴 밀크, 소듐 알지네이트, 전분(starch), 글루코스로 이루어진 군에서 1이상 선택된 보존제를 첨가하는 것을 특징으로 하는 포투랍두스 템페라타 M1021(수탁번호: KACC91627P) 동결건조 방법에 관한 것이다. 또한, 본 발명은 상기와 같은 동결 건조 방법에 의하여 만들어진 포투랍두스 템페라타 M1021 균주의 동결 건조 분말과 이를 포함하는 것을 특징으로 하는 생물 농약에 관한 것이다.