The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a blood coagulation protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer. In one embodiment of the invention the conjugation is carried out in the presence of the nucleophilic catalyst aniline. In addition the generated oxime linkage can be stabilized by reduction with NaCNBH3 to form an alkoxyamine linkage.本發明係關於使水溶性聚合物接合於凝血蛋白之氧化之碳水化合物部分的物質及方法，其包含使該氧化之碳水化合物部分與活化之水溶性聚合物在允許接合之條件下接觸。更特定言之，本發明係關於前述物質及方法，其中該水溶性聚合物含有活性胺氧基，且其中肟鍵聯在該氧化之碳水化合物部分與該水溶性聚合物上之活性胺氧基之間形成。在本發明之一具體實例中，該接合在親核性催化劑苯胺存在下進行。另外，所產生之肟鍵聯可藉由用NaCNBH3還原形成烷氧基胺鍵聯來穩定。
