FIELD: biotechnology.SUBSTANCE: according to the invention, a method for active molecules extraction from a plant substrate comprises the step of the said substrate contacting with an extraction liquid. This extraction liquid contains: an extraction gas in the gaseous state at a temperature of 23°C and a pressure of 1 atm (101.325 kPa), and an extraction solvent in a liquid state comprising or consisting of acetic acid alone or mixed with at least one of water and primary aliphatic alcohol having the formula (V) R-OH, wherein R is a C1-C10 alkyl group, preferably C1-C5, wherein the said extraction gas is selected from the group consisting of helium, neon, argon, krypton, xenon, carbon dioxide and nitrogen, or mixtures thereof wherein the said extraction gas is introduced into the said extraction solvent at a concentration of 0.1 to 10 vol % relative to 100 parts by weight of the extraction solvent. Active molecules extraction from plant substrate. Extract application for preparation of a food composition for internal use. Extract application for preparation of a food supplement for internal use. Extract application for preparation of a nutraceutical composition for internal use. Extract application for preparation of a pharmaceutical product for external or internal use.EFFECT: method allows to obtain an extract with a high yield of biologically active substances, to avoid chemical destruction of extracted molecules or modification of the original chemical structure.13 cl, 1 dwg, 10 tbl, 10 exИзобретение относится к способу экстракции активных молекул из растительного субстрата. Способ экстракции активных молекул из растительного субстрата согласно изобретению включает стадию, на которой указанный субстрат приводят в контакт с экстракционной жидкостью, причем указанная экстракционная жидкость содержит: экстракционный газ, который находится в газообразном состоянии при температуре 23°С и давлении 1 атм (101,325 кПа), и экстракционный растворитель в жидком состоянии,
