A light energy wave stem cell culture device mainly includes a box body and two light energy wave emitting units; the light energy wave emitting units are fixed in the inner space of the box symmetrically above and below, respectively, and there is a predetermined distance between Distance, and a placing platform is formed on the lower light energy wave emission unit for placing a stem cell culture instrument; each light energy wave emission unit is provided with a light energy wave emission chip inside, Light energy waves are generated when current is flowing. According to this, not only can the survival rate of stem cells during culture be improved, but also stable and rapid growth and proliferation can be maintained.一種光能波幹細胞培養裝置,主要包括一箱體及二光能波發射單元;所述光能波發射單元分別以上、下對稱方式固定於該箱體的內部空間中,且彼此間相隔有一預定距離,並在該位於下方的光能波發射單元上形成有一置放平台,以供置放一幹細胞培養器具;所述光能波發射單元內部各設置有一光能波發射晶片,可在通以電流時產生光能波。據此,不僅可以提高幹細胞在培養過程中的存活率,還可以維持穩定快速的生長與增殖。