A pure bacteria culture method of Antrodia cinnamomea comprises an acquirement step to acquire a wild Antrodia cinnamomea strain, a purification step to carry out subculture of the fruiting body of wild Antrodia cinnamomea strain in a liquid culture medium to separate and purify the Antrodia cinnamomea strain, an identification step to deliver the separated and purified Antrodia cinnamomea strain for identification to confirm that the Antrodia cinnamomea strain is a pure strain, and a pure bacteria culture step to evenly inoculate the confirmed pure strain in the identification step together with the liquid culture medium on a basswood in a smearing way. Therefore, not only in the initial stage of culture to ensure that the Antrodia cinnamomea strain is a pure bacterium, but also through directly smearing liquid culture medium enriched with pure bacteria onto the basswood, the pure strains can be grown evenly on the basswood in large area, so it could have advantage of forming bacterial colony and prevent contamination of heterogeneous bacteria.一種牛樟芝之純菌培養方法,包含一用以取得野生的一牛樟芝菌種的取得步驟、一將該野生牛樟芝菌種之子實體於一液態培養基中進行繼代培養以使該牛樟芝菌種得以分離及純化的純化步驟、一將經純化分離的該牛樟芝菌種送鑑定而確認該牛樟芝菌種為純菌菌種的鑑定步驟,及一將於該鑑定步驟中確認之純菌菌種,連同該液態培養基一同以塗抹的方式均勻接種於一椴木上的純菌培養步驟。藉此不但在培養初期確保該牛樟芝菌種為純菌,且透過富含純菌之液態培養基直接塗抹於椴木上,使該純菌菌種可均勻且大面積的於該椴木上生長,故能形成菌落優勢而防止雜菌污染。11‧‧‧取得步驟12‧‧‧純化步驟121‧‧‧固態培養步驟122‧‧‧液態培養步驟13‧‧‧鑑定步驟14‧‧‧純菌培養步驟
