КРОУЛИ Крейг (US),ДИСАВАЖ Фредерик Дж. (US),ИТОН Дэн Л. (US),ЭБЕНС Аллен Мл. (US),ЭЛКИНС Кристи (US),ХОНГО Джо-Энн С. (US),ДЖУНУТУЛА Джагатх Редди (US),ПОЛСОН Эндрю (US),РОСС Сарайн (US),СМИТ Виктория
申请号：
RU2010136303/10
公开号：
RU2010136303A
申请日:
2009.01.13
申请国别(地区):
RU
年份:
2012
代理人:
摘要：
1. Constructed anti-CD79b antibody by cysteine substitutions comprising one or more free cysteines and the sequence selected from: Sequence near Cys-mutation number in the sequence numbering by Kabat EU-numbering of SEQ ID NO EVQLCQSGAE Q5C Q5C 63 VKISCCATGYT K23C K23C 64 LSSLTCEDSAV S88C S84C 65 TSVTVCSASTK S116C S114C S118C 66 VSSASCKGPSC T120C T116C T120C 68 KFNWYCDGVEV V279C V275C V279C 69 KGFYPCDIAVE S375C S371C S375C 70 PPVLDCDGSFF S400C S396C S400C 71 SLAVSCGQRAT L15C L15C 81 ELKRTCAAPSV V114C V114C 82 TVAAPCVFIFP S118C S118C 83! 2. Constructed anti-CD79b antibody by cysteine substitutions of claim 1, wherein engineered anti-CD79b antibody by cysteine substitutions binds to a polypeptide CD79b. ! 3. Constructed anti-CD79b antibody by cysteine substitutions according to claim 1, obtained by a process comprising replacing one or more amino acid residues of a parent anti-CD79b antibody cysteine. ! 4. Constructed anti-CD79b antibody by cysteine substitutions of claim 1, wherein one or more free cysteine residues are located in the heavy chain. ! 5. Constructed anti-CD79b antibody by cysteine substitutions of claim 4 further comprising the sequence: The sequence around Cys-mutation number in the sequence numbering by Kabat EU-numbering of SEQ ID NO VTVSSCSTKGP A118C A114C A118C 67! 6. Constructed the anti-CD79b antibody by cysteine substitutions of claim 1, wherein one or more free cysteine residues are located in the light chain. ! 7. Constructed anti-CD79b antibody by cysteine substitutions according to claim 6, comprising one or more sequences selected from: The sequence around Cys-mutation number in the sequence numbering by Kabat EU-numbering of SEQ ID NO FIFPPCDEQLK S125
