An electrochemically active device is provided for collecting and retaining a biological sample with electrodes connected to conductive tracks arranged on a substrate. The device is provided with a non enzymatic and an electrochemically active receptor and the receptor is in chemical contact with the electrodes and a biological sample with a bioanalyte. The present invention also provides a holder and a point of care biosensor for the device of the present invention and a method for measuring glucose bioanalyte in a reduce volume of the biological sample. The device point of care biosensor and the method of the present invention facilitate accurate measurements concentrations of glucose bioanalytes by determining redox current values in the biological samples.