THE BIOLOGICAL RESEARCH CENTER OF THE HUNGARIAN AC;LOMA LINDA UNIVERSITY;AMERICAN GENE THERAPY, INC.;HADLACZKY, GYULA;SZALAY, ALADAR, A.
发明人:
HADLACZKY, GYULA,SZALAY, ALADAR, A.
申请号：
WO1997US05911
公开号：
WO9740183(A2)
申请日:
1997.04.10
申请国别(地区):
世界知识产权组织国际局
年份:
1997
代理人:
摘要：
Methods for preparing cell lines that contain artificial chromosomes, methods for preparation of artificial chromosomes, methods for purification of artificial chromosomes, methods for targeted insertion of heterologous DNA into artificial chromosomes, and methods for delivery of the chromosomes to selected cells and tissues are provided. Also provided are cell lines for use in the methods, and cell lines and chromosomes produced by the methods. In particular, satellite artificial chromosomes that, except for inserted heterologous DNA, are substantially composed of heterochromatin, are provided. Methods for use of the artificial chromosomes, including for gene therapy, production of gene products and production of transgenic plants and animals are also provided.
