A puncture device includes a puncture needle that punctures a puncture site of the living body and supplies thermal energy, a sensor needle having a puncture site temperature sensor that measures the temperature of the puncture site, a holder that holds the puncture needle and the sensor needle, a cartridge that receives the puncture needle, the sensor needle, and the holder, a main body to which the cartridge is attachable, a contacting unit provided with through holes penetrating the front and back surfaces thereof and provided on the surface side thereof with a contacting surface with the surface of the puncture site, and a drive unit that moves the holder such that the puncture needle and the sensor needle are projected and retracted from the contacting surface via the through holes.