A super-large scale photon capture bioreactor for water purification and aquaculture, includes a sealable three-dimensional room. Circuitous water ditches (2) at each layer inside the room. The water ditches (2) within a layer includes an inlet (12) and an outlet (13). A plurality of filter units (4) are at intervals in water ditches (2) at each layer. Float-planted plants (5) are on water surface of each filter unit, aquatic animals (6) and microorganisms (7) are underwater, and plant growth illustrating lights (8) are above float-planted plants (5) at adjustable height. A vane wheel (15) connecting a generator (16) is under an outlet (13) of an upper layer. A water inlet (12) of a next layer is under the vane wheel (15). Water to be purified is guided to an inlet (12) at an uppermost layer. The bioreactor is in low energy consumption, less occupied area, no pollution and clean production.