The present invention provides: a substrate with an antibacterial layer, the substrate being provided with an antibacterial layer that has exceptional light resistance and promptly exhibits antibacterial action an antibacterial sheet a radiography device and a touch panel. This substrate with an antibacterial layer has a substrate and an antibacterial layer disposed on at least part of the surface of the substrate, wherein the antibacterial layer contains at least one silver-containing antibacterial agent, and the silver ion content per unit area measured by an extraction test is 15-50 ng/cm2.