A sterilization system includes a first chamber, a second chamber, a sterilization module, and a pump assembly. The first chamber includes a first venting valve. The first chamber is configured to receive a first medical device. The first chamber is further operable to sterilize the first medical device. The first sterilization module is operable to dispense sterilant into the first chamber. The pump assembly is in fluid communication with the first chamber and the second chamber. The pump assembly is operable to selectively and repeatedly transfer at least some of the sterilant from the first chamber to the second chamber. The pump assembly is further operable to selectively and repeatedly transfer at least some of the sterilant from the second chamber to the first chamber.
