A medication cartridge injection assembly facilitates and tracks dispensation of medication such as epinephrine during a heart attack. The assembly includes a frame and a housing having a plurality of chambers holding cartridges of medication. A barrel is coupled to the frame and selectively alignable with each chamber. A plunger is aligned with the barrel to engage the cartridge positioned in an aligned chamber. An actuator engages the cartridge upon manipulation of the actuator. A processor is operationally coupled to a timer actuated by manipulation of the actuator. An alert mechanism is operationally coupled to the processor to provide an alert related to a time expired since a most recent dispensing of medication.