A preparation method of lipid bubbles by utilizing the principle of natural adsorption and assembly of amphiphilic lipid molecules on a gas-liquid interface comprises the steps of mixing gas-dissolved water containing free bubbles with lipid materials, and then the lipid materials are dispersed in the free gas nanobubble aqueous solution, and, in the presence of free bubbles, adsorbed and assembled on the gas-liquid interfaces so as to form lipid bubbles. The method is different from the traditional ultrasound cavitation, the mechanical force action, the novel ink jet printing method, the microchannel method and other methods for preparing bubbles.
