NATIONAL RESEARCH COUNCIL OF CANADA;HER MAJESTY THE QUEEN IN RIGHT OF CANADA, AS REPRESENTED BY THE MINISTER OF AGRICULTURE AND AGRI-FOOD;UNIVERSITY OF SASKATCHEWAN TECHNOLOGIES INC.
发明人:
WANG, HONG,FOWKE, LARRY C.,CROSBY, WILLIAM L.
申请号:
CA2329684
公开号:
CA2329684C
申请日:
1999.06.08
申请国别(地区):
CA
年份:
2013
代理人:
摘要:
The invention provides methods for using cyclin-dependent kinase (CDK)inhibitor genes, or anti-sense constructs complementary to such genes, tomodify the growth and development of plant cells and organs. The inventionprovides methods of modifying the development of plant cells and plants bytransforming plant cells with nucleic acids encoding cyclin-dependent kinaseinhibitor polypeptides, or anti-sense constructs complementary to such nucleicacids, to produce transformed plant cells, and then culturing the plant cellsor regenerating a plant under conditions wherein the cyclin-dependent kinaseinhibitor, or the anti-sense constructs, is expressed. A variety of CDKinhibitor genes, and corresponding anti-sense constructs, are disclosed foruse in a variety of plants. The nucleic acid encoding the cyclin-dependentkinase inhibitor may be operably linked to a tissue-specific promoter. Anotheraspect of the invention provides modified transgenic plants and plant tissues.The invention also provides methods of identifying nucleic acids that encodecyclin-dependent kinase inhibitors that are active in plants to modify thedevelopment of the plants.