System and method for training of regional ophthalmic anesthesia, the system includes at least one electrically conductive extraocular muscle structure, a data acquisition system (DAS), a syringe having a needle and a graphical user interface (GUI). The DAS is provided in communication with the syringe and eye structure, and configured to excite the needle of the syringe and locate the syringe having needle based on at least one of capacitance or resistance between the syringe having needle and the extraocular muscle structure. Further, the GUI receives data from the DAS and display the location of the syringe by suitable means. A method for locating a syringe having needle includes providing an electrically conductive eye structure, providing the syringe with an electrical signal and determining the location of syringe having needle based on at least one of the capacitance or resistance between the syringe having needle and the eye structure. FIG. 1