To provide a method for stably cultivating a retinal pigment epithelial cell and a production method for efficiently producing a retinal pigment epithelial (RPE) cell from a human pluripotent stem cell.SOLUTION: There are provided: a method of producing a retinal pigment epithelial cell including adhesion cultivation of a human pluripotent stem cell using a medium material to which laminin E8 fragment is coated； a cultivation method of the retinal pigment epithelial cell including a step of adhesion cultivation of the retinal pigment epithelial cell using the medium material to which laminin E8 fragment is coated； a toxicity/medicine effect evaluation method using the retinal pigment epithelial cell obtained by the above-mentioned cultivation method or production method； and a retinal disease curative medicine including the same retinal pigment epithelial cell.SELECTED DRAWING: Figure 1【課題】ヒト多能性幹細胞から網膜色素上皮（ＲＰＥ）細胞を効率よく製造する方法及び網膜色素上皮細胞を安定に培養する方法の提供。【解決手段】ラミニンＥ８フラグメントがコーティングされた培養基材を用いてヒト多能性幹細胞を接着培養する工程を含む網膜色素上皮細胞の製造法、ラミニンＥ８フラグメントがコーティングされた培養基材を用いて網膜色素上皮細胞を接着培養する工程を含む網膜色素上皮細胞の培養法、前記方法により製造又は培養して得られる網膜色素上皮細胞を用いる毒性・薬効評価法、および、同網膜色素上皮細胞を含む網膜疾患治療薬。【選択図】図１
