A method for producing retinal pigment epithelial cells which can improve retinal pigment epithelial cell differentiation induction efficiency from pluripotent stem cells and can obtain retinal pigment epithelial cells of high purity in a short period of time with a simple operation, A method for culturing retinal pigment epithelial cells that can be produced, and a toxicity / efficacy evaluation method and retinal disease therapeutic agent using retinal pigment epithelial cells useful for transplantation treatment. The present invention relates to a method for producing retinal pigment epithelial cells comprising the step of adhering and culturing human pluripotent stem cells using a culture substrate coated with laminin E8 fragment, a method for producing retinal pigment epithelial cells using a culture substrate coated with laminin E8 fragment A method for culturing retinal pigment epithelial cells including a step of adhering and culturing retinal pigment epithelial cells, a method for evaluating toxicity / efficacy using retinal pigment epithelial cells obtained by producing or culturing the retinal pigment epithelial cells by the above method, retinal diseases including the retinal pigment epithelial cells To a therapeutic agent.多能性幹細胞から網膜色素上皮細胞の分化誘導効率が改善され、簡易な操作で高い純度の網膜色素上皮細胞を短期間で得ることができる網膜色素上皮細胞の製造法、細胞を安定に増殖培養できる網膜色素上皮細胞の培養法、及び移植治療に有用な網膜色素上皮細胞を用いた毒性・薬効評価法並びに網膜疾患治療薬を提供する。本発明は、ラミニンE8フラグメントがコーティングされた培養基材を用いてヒト多能性幹細胞を接着培養する工程を含む網膜色素上皮細胞の製造法、ラミニンE8フラグメントがコーティングされた培養基材を用いて網膜色素上皮細胞を接着培養する工程を含む網膜色素上皮細胞の培養法、前記方法により製造又は培養して得られる網膜色素上皮細胞を用いる毒性・薬効評価法、同網膜色素上皮細胞を含む網膜疾患治療薬に関する。
