A pesticide-free physically pest-isolating transparent polymeric film is formed of a plastic film that has a thickness of 0.03˜1.00 mm and is perforated to include a plurality of holes. The holes have a hole size of 1˜30 mm and a distribution density of 1000˜200000 holes/M2 on the plastic film. The pesticide-free physically pest-isolating transparent polymeric film is proven as a simple but very effective way of pest control. It helps plants fully grow without being attacked by pests in a pesticide-free condition. Further, the pesticide-free physically pest-isolating transparent polymeric film is repeatedly usable, and is therefore an easily operable and economical agricultural aid ideal for use.