An assistant guiding system includes a plurality of radio frequency identification (RFID) tags, a server, and an earphone assembly. The plurality of RFID tags is located at a predetermined area, and each RFID tag pre-stores an identification number of the predetermined area. The server stores scenic spot introduction information of the predetermined area. The earphone assembly communicates with a portable electronic device of tourists, and the portable electronic device downloads the scenic spot introduction information from the server. The earphone assembly includes a reading module and a transmission module. The reading module reads the identification number from the RFID tag. The transmission module sends the identification number to the portable electronic device. The scenic spot introduction information corresponding to the identification number of the predetermined area is played or displayed on the portable electronic device.