A tool that is useful for cleaning and disinfecting the attachment surfaces of a fluid connector device used in medical applications, the tool having a housing with an opening of defined shape and a chemically treated flexible insert positionable inside the housing that substantially conforms to the inside wall of the housing and, when placed over the attachment surfaces of a fluid connector device, can be manipulated axially and rotationally relative to the attachment surfaces to contact, clean and disinfect the surfaces. Some embodiments can also be used for topical cleaning of skin or other surfaces.