An imaging device according to an embodiment of the present technology includes a first polarization section, a second polarization section, a rotation control section, and a generation section. The first polarization section irradiates a body tissue with polarization light of a first polarization direction. The second polarization section extracts a polarization component of a second polarization direction that intersects with the first polarization direction, from beams of reflection light that are the polarization light reflected by the body tissue. The rotation control section that rotates each of the first polarization direction and the second polarization direction while maintaining an intersection angle between the first polarization direction and the second polarization direction. The generation section that generates an image signal of the body tissue on the basis of the polarization component of the reflection light extracted by the second polarization section in accordance with rotation operation performed by the rotation control section.