Disclosed herein is a kind of cells, are immunosuppressant cell, the dominant negative form of the inhibitor of the cell-mediated immune response of the cell recombinant expression cell. In certain embodiments, immunosuppressant cell is regulatory T cells. On the other hand, there is provided herein regulatory T cells, recombinantly express the dominant negative form of the inhibitor for the immune response that regulatory T cells mediate. Cell can be Antigen-sensitized, and the antigen is the target of pathologic immune response relevant to immune-mediated obstacle. The method for being additionally provided with the immune-mediated obstacle using such cell therapy object in need.
