Conor L. Evans,W. Peter Hansen,Robert A. Hoffman,Petra B. Krauledat,Daniel W. Cramer
申请号：
US15556998
公开号：
US10571400B2
申请日:
2016.03.11
申请国别(地区):
US
年份:
2020
代理人:
摘要：
A method for detecting a target cell surface molecule and classifying cell types in a fluid sample. The method involves the addition of a reagent to the fluid sample. The reagent includes nanoparticles with optical plasmonic resonances, and at least one fluorescent probe. The nanoparticles are a bio-optical probe for the target cell surface molecule. Each fluorescent probe targets a cell classification marker. The method further involves the acquisition of an image using dark field microscopy and fluorescence microscopy to detect and quantify the presence or absence of any cells in the fluid sample having the target cell surface molecule or having the cell classification marker.
