A method for identifying a cluster or sub-cluster of microRNAs (miRNAs) that provides a signature profile for differentiating cells grown in one type of culture model from cells grown in another type of culture model. Also, kits and methods for evaluating, selecting, and/or characterizing tissue culture models using the miRNA profiles and a cluster or sub-cluster of miRNAs that provides the signature profile. Also, a method for identifying a putative mRNA target of a miRNA for evaluating and targeting with a drug candidate by using the cluster or sub-cluster of miRNAs and a method for selecting a culture model for use as a drug platform for screening a candidate molecule for activity against tumor cells in tumor stroma.
