Biological condition estimating apparatus capable of preventing erroneous operation in advance of the operation of equipment such as a vehicle, to improve the safety of operation of the equipment, and to provide a vehicle control device. An open-eye time acquisition section 7 for acquiring eye opening time of the drivers eyes, a variation calculation unit 8 for obtaining the variation in open-eye time acquired in eye opening time acquisition unit 7, the variation in the open-eye time acquired by the variation calculation unit 8 and a sleepiness prediction determining section 9 the biological condition of the driver based. Thus, by determining the biological condition based on a variation of the open-eye period, the arousal level is greatly reduced to the extent that make it possible to detect to the decrease in the shallow alertness, erroneous operation of the future vehicle operation occurs I estimate the.車両などの機器の運転の誤操作を未然に防止し、機器の操作の安全性を高めることができる生体状態推定装置、及び車両制御装置を提供する。運転者の眼の開眼時間を取得する開眼時間取得部7と、開眼時間取得部7で取得した開眼時間のばらつきを取得するばらつき演算部8と、ばらつき演算部8の取得した開眼時間のばらつきに基づいて運転者の生体状態を判定する眠気予知部9とを備える。これにより、開眼時間のばらつきに基づいて生体状態を判定することによって、浅い覚醒度の低下まで検出することを可能とし、将来車両運転の誤操作が発生する程度にまで覚醒度が大幅に低下することを推定する。