주식회사 파마리서치프로덕트;PHARMARESEARCH PRODUCTS CO.; LTD.;PHARMARESEARCH PRODUCTS CO., LTD.
发明人:
BAEK, SEUNG GUL,백승걸,KIM, IK SOO,김익수
申请号：
KR1020120117542
公开号：
KR1020130044195A
申请日:
2012.10.22
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: A composition for cartilage regeneration including a DNA fragment mixture separated from fish testis is provided to increase lubrication and buffering effects of cartilage when injected in a joint due to high viscosity by including a DNA fragment mixture with a molecular volume in a specific range. CONSTITUTION: A composition for cartilage regeneration comprises a DNA fragment mixture with a molecular weight of 3,000-10,000 kDa separated from fish testis. A fish is a fish of a salmon family. A manufacturing method of the composition for cartilage regeneration comprises a step of obtaining fraction liquid of the DNA fragment mixture with a molecular weight of 3,000-10,000 kDa from a gel filtration filter liquor； a step of precipitating the DNA fragment mixture by stirring after adding isopropyl alcohol or ethanol to filter liquor； and a step of dehydrating and drying the DNA fragment mixture after washing the same with an ethanol water solution or isopropyl alcohol. [Reference numerals] (AA) (First process) Step of defrosting fish testis and removing blood and foreign materials； (BB) (Second process) Step of putting refined water into the defrosted fish testis, pulverizing, and manufacturing testis pulverized liquid； (CC) (Third process) Step of mixing the testis pulverized liquid of the second process and a sodium chloride aqueous solution, reacting while stirring, and cooling； (DD) (Fourth process) Step of adding a sodium hydroxide aqueous solution into the cooled reactant liquid of the third process, adjusting into pH 10-12, stirring, filtering, and obtaining filtered liquid； (EE) (Fifth process) Step of adding hydrochloric acid into the filtered liquid of the fourth process to be pH 6.5-8.5, reacting, cooling, filtering, and obtaining filtered liquid containing a DNA fragment mixture； (FF) (Sixth process) Step of gel filtering the filtered liquid of the fifth process and obtaining fraction liquid of the DNA fragment mixture of 3,000-10,000kDA molecular w
