The United State of America; as represented by the Secretary; Department of Health and Human Service
发明人:
Eric Tran,Yong-Chen Lu,Paul Robbins,Steven A. Rosenberg
申请号：
US15514942
公开号：
US20170218042A1
申请日:
2014.10.02
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Disclosed are methods of isolating a TCR having antigenic specificity for a mutated amino acid sequence encoded by a cancer-specific mutation, the method comprising: identifying one or more genes in the nucleic acid of a cancer cell of a patient, each gene containing a cancer-specific mutation that encodes a mutated amino acid sequence； inducing autologous APCs of the patient to present the mutated amino acid sequence； co-culturing autologous T cells of the patient with the autologous APCs that present the mutated amino acid sequence； selecting the autologous T cells； and isolating a nucleotide sequence that encodes the TCR from the selected autologous T cells, wherein the TCR has antigenic specificity for the mutated amino acid sequence encoded by the cancer-specific mutation. Also disclosed are related methods of preparing a population of cells, populations of cells, TCRs, pharmaceutical compositions, and methods of treating or preventing cancer.
