Препарат экзосом - ОНКОСОМ, способ его получения, способ профилактики злокачественных новообразований и способ иммунотерапии солидных опухолей с его использованием
Голубков Сергей Петрович (RU),Сивов Игорь Геннадьевич (RU)
申请号：
RU2014149080
公开号：
RU2014149080A
申请日:
2014.12.05
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. Preparation of exosomes isolated culture K562 / i-S9 cells containing an enzyme immunoassay according to the protein in an amount of 0.9-1.5 g / 10kletok, CEA antigens in an amount of 0,045-0,075 mcg / 5T4 and 10kletok -0,03- 0.05 ug / 10kletok and ligands FasL- less than 0.005 g / 10kletok and TRAIL - less than 0.004 g / 10kletok.2. A method for producing a preparation according to claim exosomes. 1, which consists in the fact that the cell line K562 / i-S9 cultured at 36,5-37,5 ° C for 36-72 hours and maintained at 43-44 ° C, regenerated with 36 , 5-37,5 ° C for 3.5-4.5 hours, frozen and homogenized to a powder, ultraviolet ray is irradiated homogenate and precipitated cell fragments by centrifugation, the resulting supernatant was added ethyl alcohol to give its concentration of 84 ° and centrifuged at 60000g for 20-25 minutes, the resulting precipitate was dissolved in distilled water Is subjected to ultrafiltration and liofilizatsii.3. A method for preventing malignancy in a human vaccine comprising a combination of a preparation of exosomes according to Claim. 1 and P-type oligodeoxynucleotide constituting GGGGCGCCGTGATGGCGAGCGCGCC sequence of nucleotides that is administered s.c. 1 time per year in an effective amount, preferably in doses of 0.01-0.04 mg / kg kazhdyy.4. A method of immunotherapy of solid tumors, comprising a patient vaccination combination exosome preparation according to Claim. 1 and oligodeoxynucleotide P-type, which is a sequence GGGGCGCCGTGATGGCGAGCGCGCC nucleotides which is administered subcutaneously for at least 2 months, at least 1 time per week in an effective amount, preferably in doses of 0.01-0.04 mg / kg kazhdyy.5. . A method according to claim 4, characterized in that during at least part of the period of vaccination orally administered drug - reverse transcriptase inhibitor in effective doses.1. Препарат экзосом, выделенных культурой клеток K562/i-S9, содержащий по данным иммуноферментного анализа белок в количестве 0,9-1,5
