A pulse protein product having a protein content of at least about 60 wt% (N x 6.25) d.b., preferably a pulse protein isolate having a protein content of at least about 90 wt% (N x 6.25) d.b., is prepared from a pulse protein source material by extraction of the pulse protein source material with an aqueous calcium salt solution, preferably calcium chloride solution, to cause solubilization of pulse protein from the protein source and to form an aqueous pulse protein solution, separating the aqueous pulse protein solution from residual pulse protein source, optionally concentrating the aqueous pulse protein solution while maintaining the ionic strength substantially constant by using a selective membrane technique, optionally diafiltering the optionally concentrated pulse protein solution, and optionally drying the optionally concentrated and optionally diafiltered pulse protein solution.本發明係關於一種具有至少約60重量%(N×6.25)d.b.蛋白質含量之豆類植物蛋白質產品(較佳係具有至少約90重量%(N×6.25)d.b.蛋白質含量之豆類植物蛋白質離析物)，其係自豆類植物蛋白質源材料依以下步驟製得：以鈣鹽水溶液(較佳係氯化鈣溶液)萃取該豆類植物蛋白質源材料，導致來自該蛋白質源之豆類植物蛋白質溶解及形成豆類植物蛋白質水溶液，使該豆類植物蛋白質水溶液與殘餘豆類植物蛋白質源分離，視需要濃縮該豆類植物蛋白質水溶液同時藉由利用選擇性膜技術維持其離子強度實質上恆定，視需要透濾該視需要經濃縮之豆類植物蛋白質溶液，及視需要乾燥該視需要經濃縮及視需要經透濾之豆類植物蛋白質溶液。
