This invention discloses a method to produce an immune cell and a method to induce an immune effector cell. The method to produce an immune cell comprises following steps: mixing an immune cell and an immunogenic composition comprising at least two different groups of peptides or peptide pools derived from human herpesvirus-4. The immunogenic composition can further comprises adjuvants. In addition, the method to induce an immune effector cell comprises following steps: mixing antigen-presenting cell and said immunogenic composition to form a mixture, and co-culturing said mixture and lymphocytes in a proper culture medium to produce activated immune effector cells which can be used for the generation of anti-human herpesvirus-4 immune response. The culture medium can also further comprises adjuvants.本發明揭露一種生產免疫細胞之方法以及誘發免疫作用細胞之方法。生產免疫細胞之方法包含下列步驟:將免疫細胞以及免疫原組成物予以混合培養,其中免疫原組成物包含有至少兩種衍生自人類皰疹病毒第四型之胜肽或胜肽庫,且可另包含免疫佐劑。此外,誘發免疫作用細胞之方法包含下列步驟:將抗原呈現細胞細胞以及前述之免疫原組成物予以混合成培養物,以及將前述培養物和淋巴細胞共同培養於適當的培養基中,以獲取活化免疫作用細胞,而前述培養基中亦可另包含有免疫佐劑。獲得之活化免疫作用細胞可用於產生抗EBV免疫反應。1‧‧‧免疫原組成物11、12、13、14‧‧‧胜肽30‧‧‧抗原呈現細胞31‧‧‧已活化抗原呈現細胞40‧‧‧單純淋巴細胞41‧‧‧免疫作用細胞5‧‧‧患者
